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( a ) Rendering of the detection arm elements. ( b ) Zemax Opticstudio layout and beam path of optimized illumination arm, where L1, L2, L3, and L4 are 30, 80, 75, and 250 mm achromatic doublets, respectively, and ILO is <t>the</t> <t>TL20X-MPL</t> illumination objective. ( c ) The simulated light-sheet beam profile in the xz plane at the focus of the illumination objective. The inset shows an enlarged region of the illumination light sheet, highlighting light-sheet thickness and uniformity. ( d ) The cross-sectional profile through the center of the light-sheet beam profile in ( c ), where the full-width-half-maximum (FWHM) of the light sheet was found to be 0.382 µm.
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( a ) Rendering of the detection arm elements. ( b ) Zemax Opticstudio layout and beam path of optimized illumination arm, where L1, L2, L3, and L4 are 30, 80, 75, and 250 mm achromatic doublets, respectively, and ILO is the TL20X-MPL illumination objective. ( c ) The simulated light-sheet beam profile in the xz plane at the focus of the illumination objective. The inset shows an enlarged region of the illumination light sheet, highlighting light-sheet thickness and uniformity. ( d ) The cross-sectional profile through the center of the light-sheet beam profile in ( c ), where the full-width-half-maximum (FWHM) of the light sheet was found to be 0.382 µm.

Journal: eLife

Article Title: A high-resolution, easy-to-build light-sheet microscope for subcellular imaging

doi: 10.7554/eLife.106910

Figure Lengend Snippet: ( a ) Rendering of the detection arm elements. ( b ) Zemax Opticstudio layout and beam path of optimized illumination arm, where L1, L2, L3, and L4 are 30, 80, 75, and 250 mm achromatic doublets, respectively, and ILO is the TL20X-MPL illumination objective. ( c ) The simulated light-sheet beam profile in the xz plane at the focus of the illumination objective. The inset shows an enlarged region of the illumination light sheet, highlighting light-sheet thickness and uniformity. ( d ) The cross-sectional profile through the center of the light-sheet beam profile in ( c ), where the full-width-half-maximum (FWHM) of the light sheet was found to be 0.382 µm.

Article Snippet: Using the vectorial Richards & Wolf 3D optical model, the expected illumination PSF at 488 nm and maximum NA is approximately 451 nm×2240 nm (XY×Z) for the Special Optics 54-10-7 and 496 nm×2711 nm for the Thorlabs TL20X-MPL.

Techniques: